PCR M Typing: a New Method for Rapid Typing of Group A Streptococci
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چکیده
منابع مشابه
Vir typing: a long-PCR typing method for group A streptococci.
We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure for preparing DNA of sufficiently high quality from 96 samples was employed simultaneously. This ...
متن کاملTyping Group a Hemolytic Streptococci
Two methods of typing group A hemolytic streptococci have been described: slide agglutination and anti-M precipitin tests. The first, developed by Griffith (1), is more extensively used. I t has the distinct advantages of requiring small amounts of serum and culture, and of a quick reaction time when all conditions are favorable. I t has certain disadvantages: The granular growth of some strain...
متن کاملMethod for phage typing group A type 49 streptococci.
A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type 49 streptococci isolated from 10 worldwide sources were examined by this meth...
متن کاملGrowth Media for Typing Group a Streptococci.
It is frequently not possible to type group A streptococei. Only 33% of group A streptococei from Nashville children in a recent study (Quinn and Martin, Am. J. Hyg. 73:193, 1961) were typable, and there have been years when as few as 5% could be typed. This study was designed to test one possible reason for failure to type certain strains of group A streptococci, namely, inadequacy of growth m...
متن کاملNew phenotypic typing scheme for group B streptococci.
AIMS To develop a new typing system for group B streptococci based on 35S-methionine-labelled protein profiles of bacterial proteins. METHODS 377 clinical isolates of group B streptococci were examined by incorporation of 35S-methionine into bacterial proteins under strict anaerobic conditions. After sodium dodecylsulphate-polyacrylamide gel electrophoresis, autoradiography was performed. The...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2002
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.40.2.679-681.2002